Here is the family tree of the Witters of the city of Chester. However, I have not been able to fit this into our Witters yet. Silvanus Witter of the parish of Holy Trinity, city of Chester, had the following children, baptised in Holy Trinity or St. Mary's parish: John 1631 (d. 1636); Robert 1632 (d. 1633); Silvanus 1634 (d. 1635); John 1637; Frances (girl) 1639; Silvanus 1642 (married Jane Greenwood in Holy Trinity, 1665); Deborah 1644 (d. 1644). Perhaps Silvanus Witter had a brother John, who was the Mr. John Witter who died in 1678 in St. Peter and was described as an apothecary. The children of John Witter of St. Peter (likely by his wife Elizabeth, who died 1670 in St. Peter) baptised in St. Peter were Ellinor 1649 (d. 1652), John 1651 ("John son to Mr. John Witter apothecary was baptized ye 16th March [1650/1651") and William 1653. The two sons formed two main branches of the family.
Mr. John Witter, born 1651, apothecary of St. Peter parish, married in 1672 in St. Oswald Elizabeth of St. Bridgett parish, daughter of William Streete, and sister of Alice Streete, wife of Roger Cumberback, Esq. He and his wife Elizabeth had children baptised in St. Peter: Elizabeth 1674 (d. 1675); Elizabeth 1676; Jane 1677 (d. 1688); Catherine 1680 (d. 1683); Mary 1684 (married George Hockenhull, Esq. in 1705); John 1681; Anne 1685; Priscilla 1686 (d. 1688). John died in 1686 in St. Peter. His will was probated in 1686 in Plumley, in which he is described as an apothecary of the city of Chester and in which his wife Elizabeth was administratrix. In his will he mentions daughters Elizabeth and Jane (son John not having yet been born). In wife Elizabeth's will in 1708 she is described as a widow and residing in St. Peter, and mentions her only son John, her daughter Mary Hockenhull, her brother-in-law Roger Cumberback, cousin William Aken (alderman), and daughter Anne Witter, and bequeathed her two daughters an estate in Christleton. Son John, known as Mr. John Witter or John Witter, Esq., married in 1708 in St. Martin Catherine Henry, daughter of Mr. Matthew Henry, and appears to have been a merchant (described as such in both his marriage record and will). John resided in Holy Trinity but at the time of his death, in 1722, was resident in Frodsham, and was noted as a gentleman. His will of 1722 is quite extensive, and administration of the estate carried on til 1743, when it was finally settled. John and his wife Catherine appear to have only had one child, Elizabeth, born in St. Peter parish in 1709.
Mr. William Witter, born 1653, was of Holy Trinity parish and married Elizabeth Bridge in 1674. William and his wife both died in 1684. They had the following children, all baptised in Holy Trinity: John 1675 (possibly d. 1699, or is this the one born in 1637?); Richard 1677 (d. 1706); William 1681 (became a leather drosser, d. 1744, mentioned his sons Thomas and Joseph in his will), Joseph 1683 (d. 1704) and Elizabeth 1679 (married Robert Best). William junior, leather drosser, or skinner, married Ellinor Johnson in 1709 in St. Mary. He and Ellinor (d. 1738) had the following children baptised in the parish of St. Mary: John 1711 (apothecary on Bridge Street, married Alice Swarbreck, died in 1738 - estate granted to his father William); Joseph 1712 (leather drosser like his father); Mary 1713; William 1714 (d. 1736); Thomas 1715 (d. 1748); Richard 1718; Elizabeth 1718 (d. 1720); Margaret 1720 (d. 1720).
At the same time, there was another branch of Witters in the area, which appears to be unrelated. This John Whitter was ordained in Lichfield and posted to Guilden-Sutton, near Chester. Rev. Mr. John Whitter married Ellen Bushell in 1670 in St. Oswald, both of the parish of Ince. He had a son John born there in 1673 (d. 1679) and a daughter Mary born in Ince in 1675. He seems to have married a Mariam Woodcocke after the death of his first wife.